ADIPOSE-DERIVED STEM CELLS (ADSCS) PRETREATED WITH VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) PROMOTED WOUND HEALING IN RAT SKIN BURN MODEL

Stem cells are extensively used for regenerative purposes as they unspecialized capable of renewal and differentiation. Growth factors like vascular endothelial growth factor (VEGF) play a crucial role in enhancing the potential stem cells. The present study was designed to elucidate effects VEGF preconditioning accelerating adipose-derived (ADSCs) wound healing. In vivo carried out using female Sprague Dawley (SD) rats randomly divided into three groups, i.e., preconditioned ADSCs transplanted group (Pre-Tx), normal (N-Tx) control group. ADCS were isolated from SD treated with Pre-Tx At 21st day post-transplantation, completely closed. However, not fully healed N-Tx Control For further analysis, experimental area skin tissues taken all groups examined histologically. cellularity granulation thicker pre-Tx thicker, indicating rapid recovery. Furthermore, polymerase chain reaction results also confirmed down-regulation apoptotic marker caspase3, which indicates less cell death at injury site up-regulation certain IGF, sdf1α some other markers E cadherin vimentin. RT-PCR analysis revealed significant these compared groups. These suggest that pre-conditioning improves reparative potency by increasing their survival rate stimulating secretion various having involvement angiogenesis recovery damaged tissue.